Antidiabetic components from a mangrove actinobacterial culture in Vietnam

Kim Chi Hoang; Thi Hong Ha Tran; Huu Cuong Le; Thi Nhu Hang Tran

doi:10.15625/1859-3097/22730

Author affiliations

Authors

Hoang Kim Chi Institute of Chemistry, VAST, Vietnam
Tran Thi Hong Ha Institute of Chemistry, VAST, Vietnam
Le Huu Cuong Institute of Chemistry, VAST, Vietnam
Tran Thi Nhu Hang Institute of Chemistry, VAST, Vietnam

DOI:

https://doi.org/10.15625/1859-3097/22730

Keywords:

Streptomyces, actinobacteria, antidiabetic, fatty acids

Abstract

In our quest for mangrove microbial candidates with antidiabetic potential in Vietnam, actinobacterial strains were isolated and screened for inhibitory activity against carbohydrates digesting enzymes α-amylase and α-glucosidase. As a result, Streptomyces sp. strain S-X10.2 emerged as a microbe of interest since its ethyl acetate extract exhibited inhibition rates of 62.66 ± 2.50 and 58.95 ± 1.27% against α-glucosidase and α-amylase, respectively, at test concentration of 500 µg/mL. Using column chromatography integrated with bioassays, XA1 was separated from the ethyl acetate extract and appeared as the most abundant as well as the most bioactive among purified products of the chemical isolation. XA1 showed α-amylase and α-glucosidase inhibitory activities with IC₅₀ values of 294.62 and 335.22 µg/mL, respectively. Further GC/MS identification revealed the presence of saturated and unsaturated fatty acids in XA1, with oleic acid (27.70 %), palmitic acid (23.70 %), and linoleic acid (21.80 %) as the most major compounds. These results revealed evidence of bioactive fatty acids in ethyl acetate extract of an actinobacterial strain’s fermented culture. Furthermore, they contributed to drawing a new aspect in exploiting natural products from mangrove microorganism.

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